{"title":"Amino acid sensors","description":"Sensors for measure amino acids dynamics in live cells","products":[{"product_id":"bcaa-sensor","title":"BCAA sensor for branched chain amino acids","description":"Pre-made plasmids expressing BCAA sensor, highly responsive sensor for \u003cspan class=\"fontstyle0\" data-mce-fragment=\"1\"\u003ebranched-chain\u003cbr data-mce-fragment=\"1\"\u003eamino acids\u003c\/span\u003e. \u003cspan class=\"fontstyle0\" data-mce-fragment=\"1\"\u003eThe BCAA sensor displayed an \u003c\/span\u003e\u003cspan class=\"fontstyle0\" data-mce-fragment=\"1\"\u003e0.8-fold decrease and an \u003c\/span\u003e\u003cspan class=\"fontstyle0\" data-mce-fragment=\"1\"\u003e2.5-fold increase in fluorescence with excitation at 420 and 485 nm, respectively, upon leucine saturation\u003c\/span\u003e\u003cspan class=\"fontstyle0\" data-mce-fragment=\"1\"\u003e. The\u003cbr data-mce-fragment=\"1\"\u003eBCAA sensor also possessed high selectivity toward the other two branched-chain amino acids, i.e. isoleucine and valine \u003c\/span\u003e\u003cspan class=\"fontstyle0\" data-mce-fragment=\"1\"\u003e. The opposing directional changes of this sensor led to\u003cbr data-mce-fragment=\"1\"\u003ean \u003c\/span\u003e\u003cspan class=\"fontstyle0\" data-mce-fragment=\"1\"\u003e3.0-fold ratiometric change in fluorescence. \u003c\/span\u003e\u003cbr data-mce-fragment=\"1\"\u003e","brand":"Provoson","offers":[{"title":"Cytosol \/ pcDNA","offer_id":40054626615399,"sku":null,"price":700.0,"currency_code":"USD","in_stock":true},{"title":"Cytosol \/ Clone to custom vector","offer_id":40054626648167,"sku":null,"price":800.0,"currency_code":"USD","in_stock":true}]},{"product_id":"fhisj","title":"FHisJ for histine","description":"\u003cp\u003ePre-made plasmids expressing \u003cspan data-mce-fragment=\"1\"\u003eFHisJ, a series of ratiometric, highly responsive, and single fluorescent \u003c\/span\u003e\u003cspan data-mce-fragment=\"1\"\u003eprotein-based histidine sensors of wide dynamic range.  FHisJ has been validated to measure s\u003cspan class=\"fontstyle0\" data-mce-fragment=\"1\"\u003eubcellular localization and transport of histidine in mammalian cells.\u003c\/span\u003e\u003cbr data-mce-fragment=\"1\"\u003e\u003c\/span\u003e\u003c\/p\u003e","brand":"Provoson","offers":[{"title":"Cytosol \/ pcDNA","offer_id":40054627074151,"sku":null,"price":700.0,"currency_code":"USD","in_stock":true},{"title":"Cytosol \/ Clone to custom vector","offer_id":40054627106919,"sku":null,"price":800.0,"currency_code":"USD","in_stock":true},{"title":"Mitochondria \/ pcDNA","offer_id":40054627139687,"sku":null,"price":700.0,"currency_code":"USD","in_stock":true},{"title":"Mitochondria \/ Clone to custom vector","offer_id":40054627172455,"sku":null,"price":800.0,"currency_code":"USD","in_stock":true},{"title":"Nucleus \/ pcDNA","offer_id":40054627696743,"sku":"","price":700.0,"currency_code":"USD","in_stock":true},{"title":"Nucleus \/ Clone to custom vector","offer_id":40054627729511,"sku":"","price":800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0265\/9994\/8391\/products\/FHisJ.png?v=1679223855"},{"product_id":"arginine-sensor","title":"STAR sensor for arginine","description":"\u003cp\u003ePre-made plasmids expressing STAR sensor, highly responsive and highly specific sensor for arginine. \u003cspan style=\"font-size: 11.0pt; font-family: 'Calibri',sans-serif; mso-fareast-font-family: 等线; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA; mso-bidi-font-weight: bold;\"\u003eSTAR has two excitation peaks around 420 nm and 495 nm and one emission peak near 514 nm. Upon arginine binding, STAR showed an 11.4-fold increase and 1.3-fold decrease in fluorescence when excited at 485 nm and 420 nm, respectively, leading to an \u003c\/span\u003e\u003cspan style=\"font-size: 11.0pt; font-family: 'Calibri',sans-serif; mso-fareast-font-family: 等线; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA;\"\u003e~\u003cspan style=\"mso-bidi-font-weight: bold;\"\u003e1,640% ratiometric fluorescence change). Fluorescence titration studies showed that STAR had an apparent \u003ci style=\"mso-bidi-font-style: normal;\"\u003eK\u003c\/i\u003e\u003csub\u003ed\u003c\/sub\u003e\u003c\/span\u003e of ~180 μM\u003cspan style=\"mso-bidi-font-weight: bold;\"\u003e that was essentially unaffected by temperature fluctuations between 20 °C and 40 °C. STAR has high selectivity toward L-arginine but not D-arginine, also showing no apparent fluorescence changes toward or in the presence of any of the other 19 amino acids, nucleotides, glycolytic or tricarboxylic acid cycle metabolites, ornithine\/citrulline. STAR sensors has been shown useful to illuminate arginine metabolism in cells, mice, and clinical samples. \u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 11.0pt; font-family: 'Calibri',sans-serif; mso-fareast-font-family: 等线; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA;\"\u003e\u003cspan style=\"mso-bidi-font-weight: bold;\"\u003eWe also offer STAR-H sensor, which binds with arginine at a much higher affinity with \u003cspan style=\"font-size: 11.0pt; font-family: 'Calibri',sans-serif; mso-fareast-font-family: 等线; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA; mso-bidi-font-weight: bold;\"\u003eapparent \u003ci style=\"mso-bidi-font-style: normal;\"\u003eK\u003c\/i\u003e\u003csub\u003ed\u003c\/sub\u003e values of ~2.0 μM. STAR-H sensor has been successfully used to determine serum arginine level, which is a potential indicator for discriminating progression and severity of vitiligo.\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003cbr data-mce-fragment=\"1\"\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan style=\"font-size: 11.0pt; font-family: 'Calibri',sans-serif; mso-fareast-font-family: 等线; mso-bidi-font-weight: bold;\"\u003eFor precise measurement of NADH level,\u003cspan style=\"color: rgb(255, 42, 0);\"\u003e \u003cb\u003eit is very important and neccessary\u003c\/b\u003e\u003c\/span\u003e to use \u003cspan style=\"font-size: 11.0pt; font-family: 'Calibri',sans-serif; mso-fareast-font-family: 宋体; color: black; mso-font-kerning: 12.0pt; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA;\"\u003eSTAR-C\u003c\/span\u003e to correct the potential pH effects for live cell studies or in vitro studies.  We recommend customers to purchase \u003cspan style=\"font-size: 11.0pt; font-family: 'Calibri',sans-serif; mso-fareast-font-family: 宋体; color: black; mso-font-kerning: 12.0pt; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA;\"\u003eSTAR-C\u003c\/span\u003e together with your order of STAR sensors.  \u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan style=\"font-size: 11.0pt; font-family: 'Calibri',sans-serif; mso-fareast-font-family: 等线; mso-bidi-font-weight: bold;\"\u003ePromotion: For STAR sensor plasmids, customers may enjoy our buy one and get the second plasmid 50% off promotion policy. Effective during check out. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 11.0pt; font-family: 'Calibri',sans-serif; mso-fareast-font-family: 等线; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA;\"\u003e\u003cspan style=\"mso-bidi-font-weight: bold;\"\u003e\u003cspan style=\"font-size: 11.0pt; font-family: 'Calibri',sans-serif; mso-fareast-font-family: 等线; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA; mso-bidi-font-weight: bold;\"\u003eReference: \u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 11.0pt; font-family: 'Calibri',sans-serif; mso-fareast-font-family: 等线; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA;\"\u003e\u003cspan style=\"mso-bidi-font-weight: bold;\"\u003e\u003cspan style=\"font-size: 11.0pt; font-family: 'Calibri',sans-serif; mso-fareast-font-family: 等线; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA; mso-bidi-font-weight: bold;\"\u003eLi R et al.  \u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003cspan style=\"font-size: 11.0pt; font-family: 'Calibri',sans-serif; mso-fareast-font-family: 等线; mso-bidi-font-weight: bold;\"\u003eLighting up arginine metabolism reveals its functional diversity in physiology and pathology. Cell Metabolism 2024. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 11.0pt; font-family: 'Calibri',sans-serif; mso-fareast-font-family: 等线; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA;\"\u003e\u003cspan style=\"mso-bidi-font-weight: bold;\"\u003e\u003cspan style=\"font-size: 11.0pt; font-family: 'Calibri',sans-serif; mso-fareast-font-family: 等线; mso-font-kerning: 1.0pt; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA; mso-bidi-font-weight: bold;\"\u003ehttps:\/\/www.cell.com\/cell-metabolism\/abstract\/S1550-4131(24)00375-9\u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e","brand":"Provoson","offers":[{"title":"STAR \/ Cytosol \/ pcDNA","offer_id":41081221808231,"sku":null,"price":700.0,"currency_code":"USD","in_stock":true},{"title":"STAR \/ Cytosol \/ Clone to custom vector","offer_id":41081221840999,"sku":null,"price":800.0,"currency_code":"USD","in_stock":true},{"title":"STAR-H \/ Cytosol \/ pcDNA","offer_id":41081228099687,"sku":null,"price":700.0,"currency_code":"USD","in_stock":true},{"title":"STAR-H \/ Cytosol \/ Clone to custom vector","offer_id":41081228132455,"sku":null,"price":700.0,"currency_code":"USD","in_stock":true},{"title":"STAR-C \/ Cytosol \/ pcDNA","offer_id":41081230786663,"sku":null,"price":700.0,"currency_code":"USD","in_stock":true},{"title":"STAR-C \/ Cytosol \/ Clone to custom vector","offer_id":41081230819431,"sku":null,"price":700.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0265\/9994\/8391\/files\/6.png?v=1731415939"}],"url":"https:\/\/fr-biotechnology.com\/collections\/amino-acid-sensors\/amino-acid.oembed","provider":"FR Biotechnology","version":"1.0","type":"link"}