We have added a new product line, reagents for Cliva, the only long Stock's shift fluorescent RNA working on cells. Cliva can be used to monitor RNA dynamics and RNA-protein interactions in live cells and live animal.
Pepper Fluorescent RNA Collection list
Clivia Long Stock's Shift Fluorescent RNA
Sensor for metabolites
About Fluorescent RNA Technology
Fluorescent proteins are widely used in as reporters of gene expression, protein dynamics and metabolic activities. Similar to proteins, RNAs have highly complex distributions, behaviors, and functions in cells. To this end, Fluorescent RNAs (FRs) are mimicary of fluorescent proteins for RNA studies. Although there are a few FRs available, however, many of these FRs are not robust enough to trace and quantify low abundant RNAs in live cells. Peppers are a series of monomeric, multicolor FRs with much improved (one order of magnitude or even more) cellular fluorescence brightness and fluorescence turn-on ratio. Clivias are a series of small, monomeric and stable orange-to-red fluorescent RNAs with large Stokes shifts of up to 108 nm. These fluorescent RNAs allow simple and robust imaging of diverse RNA species in live cells with minimal perturbation of the target RNA’s transcription, localization, and translation. In combination with Pepper, Clivias enable the single-excitation two-emission dual-color imaging of cellular RNAs and genomic loci. Clivias can also be used to detect RNA–protein interactions by bioluminescent imaging both in live cells and in vivo. Due to its high signal-background ratio, it is also feasible to perform quantification of fluorescent RNAs in single cells or assembled cells by flow cytometry and microplate readers. These FRs provide ideal tools for live imaging of cellular RNAs.
About our highly responsive genetically encoded metabolite sensors
To monitor intracellular events, researchers have developed genetically encoded biosensors for cellular metabolites, messengers, and conditions over the past two decades. These biosensors generally consist of two basic modules: substrate-binding proteins and fluorescent proteins. From bacteria to mammals, various regulatory proteins and transcription factors specically sense intracellular biomolecules. The binding of biomolecules to the substrate-sensing protein often triggers conformational changes, which is transferred to the fused FP and affects the fluorescence intensity and/or spectra of the fluorescent proteins. FR-biotechnology provides highly responsive, state of art genetically encoded sensors such as Frex, SoNar, iNaps and Fila, which are popular for monitoring cell metabolism in live cells or in vivo.
