STAR sensor for arginine
Pre-made plasmids expressing STAR sensor, highly responsive and highly specific sensor for arginine. STAR has two excitation peaks around 420 nm and 495 nm and one emission peak near 514 nm. Upon arginine binding, STAR showed an 11.4-fold increase and 1.3-fold decrease in fluorescence when excited at 485 nm and 420 nm, respectively, leading to an ~1,640% ratiometric fluorescence change). Fluorescence titration studies showed that STAR had an apparent Kd of ~180 μM that was essentially unaffected by temperature fluctuations between 20 °C and 40 °C. STAR has high selectivity toward L-arginine but not D-arginine, also showing no apparent fluorescence changes toward or in the presence of any of the other 19 amino acids, nucleotides, glycolytic or tricarboxylic acid cycle metabolites, ornithine/citrulline. STAR sensors has been shown useful to illuminate arginine metabolism in cells, mice, and clinical samples.
We also offer STAR-H sensor, which binds with arginine at a much higher affinity with apparent Kd values of ~2.0 μM. STAR-H sensor has been successfully used to determine serum arginine level, which is a potential indicator for discriminating progression and severity of vitiligo.
For precise measurement of NADH level, it is very important and neccessary to use STAR-C to correct the potential pH effects for live cell studies or in vitro studies. We recommend customers to purchase STAR-C together with your order of STAR sensors.
Promotion: For STAR sensor plasmids, customers may enjoy our buy one and get the second plasmid 50% off promotion policy. Effective during check out.
Reference:
Li R et al. Lighting up arginine metabolism reveals its functional diversity in physiology and pathology. Cell Metabolism 2024.
https://www.cell.com/cell-metabolism/abstract/S1550-4131(24)00375-9